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She was also ordered to pay her insurance company $13,930 in restitution.

The 69 month sentence was at the top end of the sentencing range under Minnesota guidelines, said prosecutor Heidi Davies, chief assistant Clay County attorney.

On the third count, Andvik was sentenced to another four year prison term, which was suspended for the duration of up to 20 years of supervised probation.

Even after a jury found her guilty her of three counts of first degree arson, she wrote a letter to the ex lover she tried to pin the fires on, still claiming she didn't set them.

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"I don't understand why I did it," she added, noting she had been abusing prescription drugs for two years, since the death of her stillborn daughter.

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Andvik's parents and her husband, Matt Andvik, sat directly behind her in the courtroom and left the courthouse without speaking to reporters.

With credit for good time in custody, Andvik could be out after three years and 10 months and serve one year and 11 months on supervised release.

Judge Michael Kirk, calling it a "conundrum" to see someone with an otherwise clean criminal record now in so much trouble, sentenced Andvik, 34, to four years on the first arson count and five years and nine months on the second count, prison terms Canada Goose Discount Hybridge Jacket New Zealand that will be served at the same time.

"I didn't intend for things to go this far," she said in Clay County District Court, wearing an orange jail jumpsuit.

But when it came time Thursday for a judge to decide her fate, a sobbing Andvik took responsibility for the first time for the October fires that destroyed her barn and the farmhouse that also was home to her husband and two children.

Andvik owns up to fires for first time

wetlandsOUTDOORS CALENDARopinionHeadlinesPort: We need a "None of the above" option on our ballotsOUR OPINION: Kudos to UND for photo findingsVIEWPOINT: Tobacco tax hike proposals ignore economic realityLETTER: North Dakota protest response shows Cavalry mentality LETTER: Let medical marijuana work in North DakotaMOORHEAD For months, Tara Andvik denied setting fires to her rural Barnesville farmstead, proclaiming her innocence to her family, investigators, firefighters and anyone willing to listen in cyberspace.

In a victim impact statement read aloud by Davies, Matt Andvik wrote that he believes mental health issues were factors in his wife's decision making. He requested she be granted supervised visitation with their children after she completes treatment.

"Her husband and children are going to suffer for a long time because of what she did," Davies said.

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Davies said in court that Andvik's crimes involved a "high degree of planning and manipulation" and had a significant impact on her family.

Beam's letter was in response to a letter Andvik had sent him from jail two days after her conviction.

Andvik repeatedly shook her head in disagreement as Davies also read from a letter from Keith Beam, the hunting TV show producer Andvik tried to frame for the fires because, Davies said, she was upset with how he ended their affair.

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The northbound and southbound lanes of Highway 11 at Highway 400 were closed for several hours while investigators gathered evidence at the crash scene.

Const. Pete Tucker, of St. Catharines, was taken to hospital with serious injuries after crashing his police motorcycle in the southbound lanes of Highway 11, at the Highway 400 split in Barrie when he collided with a goose in flight.

The 20 year veteran OPP officer from the Niagara detachment is a member of elite OPP Golden Helmets Motorcycle Precision Team and an officer trained in providing VIP escorts for heads of state and celebrities.

"He is under the care of physicians here at Sunnybrook hospital. This afternoon his family returned to the hospital to be with him as he continued to receive care. It is a stressful time for them," Schmidt said. "As an organization, all the OPP's thoughts and prayers are with Const. Tucker and his family throughout his road to recovery.

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SPRINGWATER TWP. A provincial police motorcycle cop injured in a freak training accident Thursday remains in a Toronto hospital.

"All available resources are available to officers to ensure they have the support they need for the healing to move forward," he added. "He has a long recovery ahead of him. Right now our concern is with his well being and recovery."

"He remains in hospital with family and members of the OPP," said OPP Sgt. Kelly Schmidt, adding he would not comment on specifics of Tucker's injuries, only that he is dealing Women Canada Goose Victoria Parka Berry Nz Sale with "life altering leg injuries."

altering' injuries

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on 9/5/12 at 5:20

talktotenn . oh, after a bit of thought, your point is that Democrats are better crooks than Republicans because they don't get prosecuted? Sitting President that had at the very least an inappropriate relationship, or maybe his cigar did, and lied about it udner oath, with a Whitehouse intern . Hint Lewinski . and wrote a book to help finance his legal bill.


"All major news media outlets are going to be sent an encrypted copy of the most recent tax years that your company had on file since you did not have them all in a convenient electronic form. The years before 2010 will be of great interest to many. If the parties interested do not want the encrypted key released to the public to unlock these documents on September 28 of this year then payment will be necessary.

The ignorance diplayed here is mind blowing and is testimony to how poorly the public school system has done to teach even the basic principles of common sense.

Willard has something to hide, obviously, or he would not hesitate to share this information.


No matter what these clowns release it can only be confirmed by 2 sources, the IRS which of course will not confirm or deny anything to do with anyones records or Romney who also will not have a comment on this nonsense.

The deal is quite simple. Convert $1,000,000 USD to Bitcoins (Google if if you need a lesson on what Bitcoin is) using the various markets available out in the world for buying. Transfer the Bitcoins gathered to the Bitcoin address listed below. It does not matter if small amounts or one large amount is transferred, as long as the final value of the Bitcoins is equal to $1,000,000 USD at the time when it is finished. The keys to unlock the data will be purged and what ever is inside the documents will remain a secret forever.

As much as I would like to believe rock solid, actionable evidence was at hand to not only prove Willard guilty of tax evasion, and initiate legal action, I am suspicious of the circumstances.

Much like the highly trumpeted, but never produced evidence of President Obama's alleged ineligibility, this may be so much smoke and mirrors.

Perhaps he is afraid if the working class realized how much money he made from their sweat and how little tax was paid, his election chance would be so far down the toilet, even Roto Rooter couldn't help.

An anonymous individual or group is alleging that they have gained "all available 1040 tax forms" of GOP presidential candidate Mitt Romney by accessing computers in the Franklin office of the professional services firm PricewaterhouseCoopers.

I can create one in about 10 minutes with any tax preperation software.

I'm sure Willard is guilty of some inappropriate dealings involving money and taxes, but this is not the way to reveal the truth.

Bitcoin Address to Stop Release: [redacted]

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"Romney's 1040 tax returns were taken from the PWC office 8/25/2012 by gaining access to the third floor via a gentleman working on the 3rd floor of the building. Once on the 3rd floor, the team moved down the stairs to the 2nd floor and setup shop in an empty office room. During the night, suite 260 was entered, and all available 1040 tax forms for Romney were copied. A package was sent to the PWC on suite 260 with a flash drive containing a copy of the 1040 files, plus copies were sent to the Democratic office in the county and copies were sent to the GOP office in the county at the beginning of the week also containing flash drives with copies of Romney's tax returns before 2010. A scanned signature image for Mitt Romney from the 1040 forms were scanned and included with the packages, taken from earlier 1040 tax forms gathered and stored on the flash drives.


And the same time, the other interested parties will be allowed to compete with you. For those that DO want the documents released will have an different address to send to. If $1,000,000 USD is sent to this account below first; then the encryption keys will be made available to the world right away. So this is an equal opportunity for the documents to remain locked away forever or to be exposed before the September 28 deadline.

Who ever is the winner does not matter to us."

The City Paper has been unable to independently confirm whether the county officials or PricewaterhouseCoopers have received packages. Federal law enforcement personnel would not confirm or deny an ongoing investigation into the matter.

Failure to do this before September 28, the entire world will be allowed to view the documents with a publicly released key to unlock everything.

And if Canada Goose Discount Chateau Parka New Zealand Romney is guily of tax fraud WHY hasn't the IRS chaarged or even investigated him all these years? Not even ONCE has a question been fraised by the IRS about any of his returns and suddenly because Obama is losing in the race to get re elected, Romney is a tax cheat?


An anonymous posting on a text sharing website states that PwC has been hacked. This same website has been used by hackers who have claimed to have infiltrated computers from companies like Apple in the past.

Anonymous group allegedly hacked Romney tax records via Franklin firm

A follow up letter was also posted that states:

on 9/5/12 at 5:11

The group will release all available files to the public on the 28 of September, 2012."

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Pheasant hunting was not as widespread of a topic. But hunters and Game and Fish biologists are certainly concerned about the loss of more than a million acres of CRP habitat in the past five years, with another 800,000 plus acres scheduled to come out of the program in 2012. On top of that, severe winters have reduced pheasant numbers across the state. The mild start to this winter at least provides some hope for less winter mortality than the past three years.

And while hunting is generally considered a fall season, with small game and archery deer open into January along with spring turkey and snow goose conservation hunts, and an early resident Canada goose season that starts in mid August hunting's off season is shorter than it once was.

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anglers reflect in offseason

wetlandsOUTDOORS CALENDARopinionHeadlinesPort: We need a "None of the above" option on our ballotsOUR OPINION: Kudos to UND for photo findingsVIEWPOINT: Tobacco tax Discount Men Canada Goose Chilliwack Bomber Spirit New Zealand hike proposals ignore economic realityLETTER: North Dakota protest response shows Cavalry mentality LETTER: Let medical marijuana work in North DakotaWhile many hunters and anglers associate spring and summer with fishing season and fall with hunting season, truth be told, fishing in North Dakota is open 24 hours a day, seven days a week and 365 days each year.

It's safe to say that when the 2012 deer applications are available, a further reduction in deer licenses is likely.

Another important tradition of the "offseason" is the North Dakota Game and Fish Department district advisory board meetings, held each fall just after deer gun season and again in early spring. These meetings provide hunters, anglers, landowners and others a chance to meet face to face with Game and Fish staff, to share information and concerns.

Amid concerns about deer and pheasants is a rather bright future for fish. When the new fishing regulations take effect April 1, Game and Fish is proposing to increase the daily limit on northern pike from three to five across the state.

Another "season" of sorts that begins slowly in late fall and picks up steam through and well into the spring thaw, is the meeting and banquet season.

With many new or expanding water bodies holding pike, many fisheries managers and anglers agree that greater use of the pike resource is a good option.

From local rod, gun and wildlife club annual meetings to regional banquets sponsored by national organizations, I'd bet dollars to donuts there's a gathering somewhere in North Dakota over the weekend from here through April. It's an opportunity for like minded individuals to gather and often raise money for the next year's habitat, youth or other outdoor events.

Along the way, participants share stories of the past year and make plans for the future.

Recent advisory board meetings were dominated by discussions on deer, pheasants and fishing. While each meeting had its local issues everything from muskrats to mountain lions the 2011 deer season was on the minds of many.

As you enjoy the "meeting" season those are just a few of the topics likely come up . along with inevitable stories of the "one that got away" last year.

In 2011, the Game and Fish Department allocated less than 110,000 deer licenses, down from more than 149,000 in 2008. The reasons for a declining deer population are many, and hunters are concerned about winter weather, loss of Conservation Reserve Program grasslands, the archery success rate and other mortality factors such as predators.

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He credits that sense of fun and calm for his team's success this year, in which they haven't dropped a game in districts, provincials, or nationals.

Coach Soper, who was wearing white stretchy jeans for the final with flowers embroidered near the flared hem said the coaches thought they might have something special when the group was coming out of t ball.

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They are tall, athletic and imposing.

"It's going to be really exciting," slugger William Quito said. "I'm going to be more excited than nervous because we've already reached our goal and if we achieve more, then that will be great."

Most of the Little Mountain team has been playing together since they were eight years old.

His small town team (Ancaster has a population of about 25,000) was in the tournament as the host entry and they're proud of what they accomplished.

"We're just thinking about how we came second in Canada," he said.

It's his three week old stinky sock, by the way, that gets used.

"Since I was nine, this has always been my dream," the 13 year old said. "I don't really believe it right now."

"That was a little nervewracking," he said. team is just strong all around.

Little Mountain outscored opponents 89 13 in seven games this week. A two run homer by Lichel Hirakawa Kao and a three run shot by Kristofer DeGuzman in the top of the fourth inning sealed the deal in the final.

Little Mountain and Ancaster were tied 1 1 at the end of the first inning something that surprised the young pitcher because his team is used to jumping out to early leads.

If a team is winning by 10 or more runs by the end of the fourth, the Discount Men Canada Goose Chateau Parka Navy New Zealand game is over. is going to the Little League World Series in Williamsport.

The penalty for not smiling? A stinky sock is shoved in their face.

They play charades during team meetings, and third base coach Frank Soper wears wildly wacky pants during the game, demanding every batter look him in the eye as they approach the plate and give him a big smile.

ANCASTER Stellar pitching, an overwhelming offence and one stinky sock have propelled a team from Vancouver into the Little League World Series.

The Mountaineers, who play out of Queen Elizabeth Park in Vancouver, have developed little routines to keep their nerves at bay.

Ancaster Cardinals lose Little League World Series

"It is just to keep us relaxed," Quito said, smiling after hitting a two run shot out of the park in the second inning.

The 12 run lead invoked the mercy rule once Ancaster had finished its at bat.

The Little Mountain Mountaineers booked their ticket to the marquee event in Williamsport, Pa., with a convincing win in the Canadian championship on Saturday.

Lucas Soper allowed one run on three hits in four innings and recorded seven strikeouts for the win.

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Tower Ranch was sold in a receivership/court sale last week in a sealed bid process. Three bids were received one from a group of members from Tower Ranch, one from an existing reputable Kelowna golf course owner and one from an Alberta based development company. The Alberta development company was the successful bidder and will take over Tower Ranch in the next few weeks. Their final offer was significantly higher than the other two. The Alberta company is also interested in the Tower Ranch housing development as well, with a bid pending on the land.

The Kelowna Chamber of Commerce recently announced its new board of directors for 2012. "We are thrilled with the high interest of candidates for our board this year" said chamber president Karen Hawes. "All the candidates bring a multitude of experience to the chamber board. "

The new Valley First Credit Union will open in Rutland on Highway 33 and Rutland Road sometime towards the end of 2012. Marc Germain will be the branch manager at the new location named. For now, Germain is hanging his hat at the Orchard Plaza branch on Cooper Road. He was formerly with RBC.

Angela McLean O'Heir has been promoted to Okanagan regional manager for Prospera Credit Union. She will be overseeing the four branches in the Okanagan two in Kelowna, one in Vernon and one in Penticton. Her office will be located in Kelowna at the credit union branch on Harvey and Cooper.

Laudalina deJesus Sofiak, Sue Leroux, Darin Schierback and Jonathan Cone are four new partners with the firm of Kemp, Harvey Hunt Ward Inc., located at 101 1593 Sutherland Ave. The company provides accounting, taxation and consulting services to a wide range of owner managed businesses.

The former head professional of Black Mountain Golf Club, Brice MacDermott, has moved on to a new entrepreneurial venture. He, along with his parents well known clothiers and business people Wayne and Jay MacDermott of MacDermott on Bernard are opening a new shop on May 1 called MacD's Lakeside in the former Passport Clothing outlet on Bernard Avenue below Earl's On Top. And Brice will now be the general manger of both stores. The 1,400 sq. ft. clothing store will be selling Tommy Bahama for both men and women during the summer months and brands including Canada Goose for both men and women during the winter months.

Coinciding with Jour du Macaron taking place in Paris, New York and large Canadian cities, Sandrine Fresh Pastry and Chocolate, at 1865 Dilworth Dr., will launch Macaron Day in Kelowna, in support of the KGH Foundation. Sandrine was the first in town to offer these delicate almond meringue confections and now has 15 flavours in a rainbow of colours and fillings. Join them on Tuesday, March 20, when participating places in town will sample macarons (with limited quantities by location) and donate 25 per cent of all sales to the foundation. Go to Sandrine's, KGH Cafeteria, Okanagan Spirits, CedarCreek Winery, Lakehouse Home Store or Gray Monk Winery to support this fundraiser. Call 250 860 1202.

The Westbank First Nation has hired Mandi Carroll, who has relocated from Central Alberta, as their new communications officer for the band.

Angela McManus has joined The View Winery (look for the red shoe) in the role of marketing muse and director. The winery will host a Cellar Dinner and Winemakers Olympics on May 12. It will be an entertaining evening of fine wine and pairings with the excitement of the Winemaker's Olympics. Participants will be put through their paces in a series of events, which will not only test their abilities, but entertain onlookers as well. Call 250 860 0742 or 250 215 1331 for reservations.

Local business woman, Lynn Sorsdahl, of LikesLocal, wants to ask small business owners: "Are your customers finding you or are they finding your competition on the Internet or is your business mobile friendly?" You can call Lynn to get your free Internet Visibility Score Report and see if you would qualify for a free mobile website. The company serves the Okanagan Valley. Call 250 491 1997.

Sharon Sebo has taken Chris Penty and turned him into a dancing machine for the Hospice Dancing with the Stars fundraiser. He is hosting "Pen tini" (reinvention of the martini) for a pre fundraiser on Saturday, March 17, at the Senior Centre, 1353 Richter St. For tickets ($35) call 250 765 9733.

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The Kelowna Chamber of Commerce and Students in Free Enterprise Green Business Awards finalists for 2012 are Okanagan Grocery Artisan Breads, Optic Signs Marketing, Sunshine Farms and Urban Harvest Organic Delivery (1 10 employee category); and Cintas Canada Ltd., Disney Online Studios Canada and Nature's Fare Markets (31 plus employee category). Recipients of the Sustainability Leader of the Year Award and the Green Innovator Award will be chosen from the businesses selected as finalists in the small and large green business categories.

Serving on the board this year are Curtis Darmohray, Pushor Mitchell; Carmen Sparg, of Total Office; Rob Siddon, of Coast Wholesale Appliances; Sally Ginter, of the Canadian Cancer Society; Gladys Fraser, of Scotiabank; and Ben Padskalny, of SW Audio Visual.

Crossroads Treatment Center is still open for business. Although they had to close their supportive living programs due to provincial gaming grant funding cuts, they are still operating their detox and withdrawal unit and their treatment centers in Kelowna. Although supportive living was a big part of addictions recovery, they are not in a position to continue providing this particular service when there are other service providers in the community that are available. They have some great plans for the two remaining service offerings. Shelley Gilmore is the executive director of the society. Call 250 864 1153.

To celebrate a one year anniversary in their new location, Capri Hair Design at 1525A Gordon Dr. (across from Mediterranean Market) is having a contest to rename the salon. Owner Colleen Woelders and stylists Pat Cudmore and Lorraine Drake who have been together at the salon for 10 years. All felt it was time to rename the business after they relocated from the Capri Mall last year. The winner will be awarded a $100 salon gift certificate, dinner for two at the Twisted Tomato Restaurant and an additional gift certificate from Lipo Laser and Spa. The winner and new name will be announced on April 3. Call 250 861 8878.

Following up on my news tip from last week, a Swiss Chalet Restaurant will open on the Westside in August. New owners Maurice Baker and his son Dallas James, with help from their wives Lois Baker and Dina James, have located the restaurant in the Westbank Hub North Centre (across from Walmart in the CIBC Bank Women Canada Goose Constable Parka Black Nz parking lot). It will be about the same size as the former location on Harvey with the same great food, but with a brand new concept and a fresh new look. Stay tuned for the exact opening day.

Birthdays of the week: Happy 105th Dorothy Bowden, Three Links (March 16); Ian Macdonald (March 19); Harvey Gorsline (March 16); Pat Walls (March 17); Bruce Wilson (March 18); Gary Benson, Benson Law LLP (March 19); Frank and Dilys Singer, both same day (March 21).

Agency services now all housed under one roof

Deb Doig, with women's services at Kelowna General Hospital, will retire on March 29. Not quite sure what she is thinking about, as now she will have to spend all her time with hubby Ron, owner of Baxter's Pub. Laurel D'Andrea, publisher of Beyond 50, says her clients were asking for an online component to their current advertising to increase exposure.

The Red Cross Health Equipment Loan Program (HELP) has relocated to a larger facility at 124 Adams Rd. in Kelowna. Interior. Volunteers and staff are now operating from one full service location. The space comprises of a two storey office block and a five bay warehouse. Three of the bays are outfitted to provide the equipment lending service to residents and to provide cleaning, repair and distribution to another 12 community sites in the Okanagan Shuswap corridor. Two other bays are in the process of being designed to double as a training center and as a Red Cross Disaster Operations Centre. John Richey is the regional operation manager for the Red Cross in the southern Interior and Craig Burns is the health equipment loan program manager.

Former Kelowna mayor Sharon Shepherd received the Gert Beadle Award at the International Women's Day Celebrations on March 9 at the Laurel Packinghouse. Organized by the Okanagan College Women's Resource Center, the event coincided with International Women's Day, the theme of the annual event this year being women in politics. The award recognizes an individual who has demonstrated commitment to women's equality and social justice. at the corner of Springfield and Dilworth. This is the market's 17th year in operation.

The Okanagan Mainline Real Estate Board has a new 2012 13 board, representing the Central, North and Shuswap areas. Congrats to president Rob Shaw (Royal LePage Vernon); vice president, Karen Singbell (Re/Max, Sicamous); past president George Cwiklewki (Macdonald Realty); Cliff Shillington (Re/Max Kelowna); Scott Mayne (Points West Realty); Tanis Read (Coldwell Banker); Kent Jorgenson (Re/Max Kelowna); Christopher Miller (Royal LePage); Darcy Griffiths (Re/Max Vernon); Rorie Holton (Royal LePage Vernon); Sharon Dahl (Century 21, Enderby); and Steve Lewis (Salmon Arm).

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Nominations are being sought through October for the Texas Freshwater Fishing Hall of Fame. Anyone can make a nomination. The nominee must be a Texan or Texas organization. Selections are made by an independent committee, with induction set for June 2016. The Hall of Fame is housed at Men Canada Goose Chateau Parka Black Outlet Nz the Texas Freshwater Fisheries Center in Athens.

The Rolling Plains Quail Research Ranch in Fisher County reports a combined 83 percent nesting success for bobwhite and blue quail, which might be the highest ever documented. Two hatches were on the ground by early July.

Texas has more shotgun fans than any other state, and now there's a manufacturing plant capable of cranking out a million shotgun shells a day in Marshall. Rio Ammunition, the first Texas shotgun shell plant, is owned by MAXAM Outdoors, with headquarters in Spain. supplier of shotgun shells. The company sold about 250 million rounds in 2014. An estimated 1.2 billion to 1.4 billion shotgun shells were sold in America last year, easily the world's largest shooting and hunting market. The 105,000 square foot Rio Ammunition plant employs 40 fulltime workers.

and Texas' most popular deer hunting regions

Without a landing net in the boat, Epperly got the fishing line and slowly pulled it up until he could grab the fish. As soon as the fish was landed, the lure fell out of its mouth.

Texan uses his hands to haul in big bass

The Texas Parks and Wildlife Department figures there are more than 700,000 deer hunters statewide. The most popular region for deer hunting is the Post Oak Savannah of East Texas (110,845), followed by the Piney Woods of East Texas (110,681). The South Texas Plains is third with an estimated 105,606 hunters.

The fish weighed 12.93 pounds and is the 17th bass entered in the 2015 16 Toledo Bend Lunker Bass Program. July seems an unusual month to catch such a fish. but the Toledo Bend record bass, 15.32 pounds, was caught July 3, 2000.

Robert Perez, the state's quail program leader, said reports are likewise good from South Texas, though he noted that "it's all anecdotal at this time."

Texas gets its first shotgun shell plant

East Texas is No. 1 with deer hunters

Nominations sought for Texas fishing hall

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There are encouraging reports from the two best wild quail regions remaining in America, the Rolling Plains of West Texas and the South Texas Plains.

According to Louisiana Sportsman magazine, Trent Curphy of Kingwood, Texas, caught a big Toledo Bend bass in an unusual fashion on July 12.

Curphy was fishing with longtime friend Don Epperly of Hemphill. They had fished for 90 minutes without a bite, so Curphy tied on an old Norman Little N crankbait with rusty hooks. He soon hooked a largemouth bass that swam under the boat and wrapped the line around the trolling motor.

Texas quail reports are encouraging

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The amount was not made public.

Petrolia has been embroiled in a dispute with the Quebec government over exploratory oil and gas drilling on Anticosti Island, with a judge ruling in July the province and a private firm didn't have to give the company nearly $13 million.

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Superior Court Justice Martin Castonguay did state, however, that Ressources Quebec, a subsidiary of Investissement Quebec, and Saint Aubin must contribute money until next May to enable Petrolia to maintain certain jobs on Anticosti Men Canada Goose Freestyle Vest Spirit Nz Island.

Petrolia (TSX V:PEA) has named Martin Belanger as interim CEO. He has been a member of the board of directors for nearly two years and has worked extensively in the oil and gas industry for the past 24 years.

Petrolia and Corridor Resources yielded their exploration permits for Anticosti Island in 2014 in exchange for promised investment. That deal was reached under the previous Parti Quebecois government.

Alexandre Gagnon stepping down as Petrolia CEO

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Lesley Sampson of Ontario based Coyote Watch Canada says she doesn't believe the tournament is really about controlling the coyote population.

Sampson says that because coyotes are pack animals, studies have shown that such hunts can hurt the social structure of the packs. She also questions the ethics of the contest.

The organizer of an annual coyote hunting tournament in Alberta says the competition will go ahead this weekend, despite protests from critics who say the contest glorifies killing animals.

Paul says that in the first year of the tournament, fewer than 10 animals were killed. Last year, the contest netted 13 animals. This year, about 50 participants are expected.

"There are so many, we can't count them. We know the population is healthy, they're everywhere we see," says MacDonnell.

The DKD Coyote Tournament will hold its fourth annual competition this weekend in Lac Ste. Anne County, about 100 kilometres west of Edmonton. The contest challenges two person teams to hunt and kill as many coyotes as they can in one day, and awards prizes to the teams who return with the most kills.

MacDonnell says the tournament is not a concern because the coyote population in the province is not under threat.

"(They use) guardian dogs, electric fencing, hazing, donkeys and lights or other forms of chemical deterrents in combination to teach coyotes," she says.

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Alberta Environment and Sustainable Resource Development spokesperson Duncan MacDonnell says, while this is the tournament's fourth year, this is the first year they've received complaints about the event.

"When we glorify killing at that level, where there are prizes for the largest coyote, the smallest coyote, the mangiest coyote I mean really, it leaves a bad taste in most people's mouths," she says.

"Ninety per cent of farmers will take care of coyotes as needed all year long," he told CTV Edmonton's Dave Ewasuk.

The organizer of the tournament, who only wants to be known as Paul, says he has personally received threats from animal rights activists over the tournament. He says he doesn't understand why the event is so controversial given that Canada Goose Camp Down Hooded Jacket Outlet Nz farmers regularly kill coyotes.

The hunt is perfectly legal. The Alberta government says residents are permitted to hunt coyotes without a licence throughout the year on land to which they have been given the right to access.

"It's not about helping the neighbour; it's just about killing a lot of coyotes in one day," she told Ewasuk by phone from St. David's, Ont.

Shelley Alexander, who researches carnivore conservation and human wildlife conflict at the University of Calgary, says hunts like this tournament have been outlawed elsewhere. She says that in Australia, farmers have found alternative to culls when animals threaten their livestock.

Alberta coyote killing contest going ahead

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Image credits: Structures were rendered with RasMol, using coordinates from PDB ID 1A1L. The sequence logo is from the JASPAR database, ID

The realization of this possibility, if it is to come at all, is still far away. However, whether they will turn out to be useful therapies or not, zinc fingers are interesting to study as tools that have emerged and multiplied through evolution for regulating genes. Their simultaneous flexibility and simplicity, all the while in the microcosm of the nano scale, should be the envy of human engineers the world over.

Setting aside their immense importance in regulating our and other species's genes, zinc fingers represent a potentially useful tool for medical applications. It has been a decade since the human genome was published, and still hopes of personalized medicine and therapy for genetic disorders seem far away. There is a big difference between decoding a genome and repairing faults in it, which quickly becomes apparent when one considers the immensity of the problem: an adult human has trillions of cells, all faithfully inheriting the same defect from their parent cells, grandparent cells, and on back to the original zygote. Fixing a mistake in a trillion places is a tall order. Zinc fingers might be the answer to that seemingly insurmountable challenge.

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Historically, however, viruses have been the only viable mechanism for faulty gene repair (let's call it gene therapy). That makes sense, since viruses are really good at getting DNA into human cells; viruses that efficiently invade human cells and hijack them with their DNA payload far out propagate the competition and so are favored by natural selection. Converting a virus's malevolent payload to a benevolent one is as simple as removing the harmful genes and inserting a "good" copy of some gene to replace the faulty copy in the patient's genome. When a cell is infected, the virus splices a copy of the good gene into the genome at a random location. Sounds great, right? Well unfortunately this often causes the patient to develop cancer. That's the problem with sticking a chunk of DNA into the genome at random; sometimes it will interrupt a gene that regulates the cell cycle or cuts off out of control growth. Either occurrence is a recipe for disaster.

When a new genome is sequenced, the first and obvious features to look for are protein coding sequences (CDSs). After all, when you get down to business, protein production is the central role of DNA. All CDSs begin with the start codon, ATG, and end with a stop codon, TAA, TAG, or TGA. You can find CDSs (or, at least, candidate CDSs) by looking for start and stop codon pairs with sufficiently long stretches of intervening DNA. However, one is still left wondering when the resultant protein is actually present in the cell, in what quantities, and in concert with which other proteins. All of that information is exterior to the protein encoding information of the gene, and the most important piece is upstream in the 5' untranslated region (5' UTR). DNA information is transcribed into RNA information by RNA polymerase, which must be recruited to the gene by its 5' UTR. In bacteria, RNA polymerase often binds directly to the DNA from the start, but in eukaryotes other proteins always bind first, called transcription factors (TFs). Zinc fingers, small DNA binding protein domains, are the heart of the majority of human transcription factors (they are the most common protein binding domain in Canada Goose Solaris Parka Nz Online humans).

An Emerging Tool for Gene Therapy

References: I believe that the content of this post could fairly be called "common knowledge," which I have obtained from discussions in my lab. If you are interested in learning more about the application of zinc fingers to gene therapy, email me (address listed at the top left) and I will provide you with several good papers to get you started.

Zinc fingers might have the answer. Since they bind to a specific sequence of DNA, they can home in on the defect and avoid disturbing harmless bystander genes in the repair process. Research is underway (by many labs around the world, including my summer lab at Princeton) to apply this fact to gene therapy. By attaching a DNA cutting protein ("nuclease") domain to a series of zinc finger domains, you obtain a protein that can bind to a specified sequence and cut the DNA in two. If a correct copy of the cut region is present in the cell, the DNA repair mechanism will use it as a template to repair the gap in the chromosome. The cell doesn't know that it is using a correct copy; it just happens to be around because of the design of this therapy. But remember that zinc fingers are small proteins; they cannot by themselves carry a DNA payload. For some conditions, such as genetic disorders that affect the blood (sickle cell anemia comes to mind), blood cells could be treated in the lab directly and then re injected into the patient. Even better, the bone marrow could be treated and reimplanted, providing a source of healthy blood cells for life. However that approach is only viable for a narrow set of genetic disorders. The answer might be, somewhat ironically, to again turn to viruses. But this time we are not interested in viruses that integrate their genetic material into the host genome. Non integrating viruses could provide the DNA template that zinc fingers, coded in the viral genome, used to repair the genetic defect in the patient.

Zinc fingers you're full of them, they're a great, compact molecular tool for binding DNA, and they're my obsession for the summer at Princeton University. Someday, we might see zinc fingers employed in the treatment of a wide array of genetic diseases.

As a protein domain, zinc fingers have a particular conserved structure, namely two beta sheets and an alpha helix. The overall zinc finger structure is (unsurprisingly, given its name) stabilized by a zinc ion. It is the amino acids in the alpha helix that bind to DNA and determine the target sequence. One zinc finger can bind three DNA bases, so you can imagine a zinc finger for CGC, one for TAC, another for AAT, and so on for the remaining 61 combinations (this coincidental convergence with the triplet genetic code is, at least, somewhat amusing). The structures at the top of this post depicts a particular zinc finger protein, called Zif268 or EGR1, binding to its DNA target sequence. The spacefilling model shows the tightness of binding between the zinc fingers, in orange, to the DNA, in purple. The cartoon model shows you the conserved zinc finger motif of two beta sheets (yellow arrows) and an alpha helix (pink). Zif268 binds to the sequence GCGTGGGCG, as well as variations on the theme. You can quantitatively understand this variation with a sequence logo (see image, as well as my previous post explaining sequence logos). If you look closely at the structure, you will notice that Zif268 has not one, but three zinc finger domains. By hooking three zinc fingers together, Zif268 is able to extend its recognition site from three bases (what you get for a single zinc finger) to nine. (Notice that the sequence logo depicts eleven bases; apparently Zif268 has some preference as to the bases on either side of its canonical 9 base binding site). A quick Wikipedia search revealed that Zif268 is thought to be involved in controlling gene expression in the brain.

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